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Eurofins dna sequencing service
(A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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1) Product Images from "Plasmid Curing of Pseudoalteromonas haloplanktis TAC125 Using Homologous Recombination and PTasRNA Gene Silencing"

Article Title: Plasmid Curing of Pseudoalteromonas haloplanktis TAC125 Using Homologous Recombination and PTasRNA Gene Silencing

Journal: Bio-protocol

doi: 10.21769/BioProtoc.5687

(A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus DNA ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense sequence (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
Figure Legend Snippet: (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus DNA ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense sequence (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).

Techniques Used: Agarose Gel Electrophoresis, Plasmid Preparation, Negative Control, Cloning, Sequencing, Marker

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Amplification:

Article Title: Cefmetazole, flomoxef, and meropenem are effective against planktonic cells but not biofilms of extended-spectrum β-lactamase-producing Escherichia coli.
Article Snippet: .. Seven loci (adk, fumC, icd, purA, gyrB, recA, and mdh) were amplified using PCR and sequenced using a DNA sequencing service (Eurofins Genomics, Tokyo, Japan). ..

Article Title: Identification and Characterization of Antiyeast Organic Acids Produced by Lactiplantibacillus plantarum 3121M0s Derived from Mongolian Traditional Fermented Milk, Airag
Article Snippet: .. The resulting PCR amplicon was extracted using MagExtractor DNA Fragment Purification Kit (Toyobo Co., Ltd.; Osaka, Japan) after agarose gel electrophoresis, and then submitted to the DNA sequencing service at Eurofins Genomics K.K. (Tokyo, Japan). ..

Polymerase Chain Reaction:

Article Title: Cefmetazole, flomoxef, and meropenem are effective against planktonic cells but not biofilms of extended-spectrum β-lactamase-producing Escherichia coli.
Article Snippet: .. Seven loci (adk, fumC, icd, purA, gyrB, recA, and mdh) were amplified using PCR and sequenced using a DNA sequencing service (Eurofins Genomics, Tokyo, Japan). ..

Article Title: Bacterial N-demthylases as biocatalysts for the production of methylxanthines
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Article Title: A new species of Athelges ( Crustacea , Isopoda , Epicaridea , Bopyridae ) from Japan, with morphological and DNA barcode data
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DNA Sequencing:

Article Title: Cefmetazole, flomoxef, and meropenem are effective against planktonic cells but not biofilms of extended-spectrum β-lactamase-producing Escherichia coli.
Article Snippet: .. Seven loci (adk, fumC, icd, purA, gyrB, recA, and mdh) were amplified using PCR and sequenced using a DNA sequencing service (Eurofins Genomics, Tokyo, Japan). ..

Article Title: Identification of essential residues for the biosynthesis of momilactone intermediate diterpene in wild rice <i>Oryza officinalis</i>
Article Snippet: .. Nucleotide sequences of all constructs were confirmed by DNA sequencing service (eurofins genomics, Tokyo, Japan). ..

Article Title: Bacterial N-demthylases as biocatalysts for the production of methylxanthines
Article Snippet: Isopropyl-β-D-thiogalactopyranoside was purchased from Indofine Chemical company (Hillsborough, NJ). .. The PCR primers were bought from Eurofins Genomics (Louisville, KY), which also provided DNA sequencing service. ..

Article Title: A new species of Athelges ( Crustacea , Isopoda , Epicaridea , Bopyridae ) from Japan, with morphological and DNA barcode data
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Article Title: Plasmid Curing of Pseudoalteromonas haloplanktis TAC125 Using Homologous Recombination and PTasRNA Gene Silencing
Article Snippet: MilliQ water purification system (e.g., Merck Millipore Q-Paks System, catalog number: ZMQS6000Y) 6. .. DNA sequencing service (e.g., Eurofins Genomics, Ebersberg, Germany) 7. ..

Article Title: Identification and Characterization of Antiyeast Organic Acids Produced by Lactiplantibacillus plantarum 3121M0s Derived from Mongolian Traditional Fermented Milk, Airag
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Construct:

Article Title: Identification of essential residues for the biosynthesis of momilactone intermediate diterpene in wild rice <i>Oryza officinalis</i>
Article Snippet: .. Nucleotide sequences of all constructs were confirmed by DNA sequencing service (eurofins genomics, Tokyo, Japan). ..

Stable Transfection:

Article Title: Direct tensile force activates Adgrl3 in a tethered agonist-dependent manner
Article Snippet: Plasmid construction was made either by restriction enzyme digestion followed by ligation or by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB, Cat. No. E2621L). .. All sequences were confirmed with Eurofins Genomics DNA sequencing service. cDNAs encoding SNAPf-tagged Adgrl3, Adgrl3-TM1, Adgrl3 LM>AA, and Adgrl3 T>G, were inserted into pcDNA5/FRT/TO (Thermo Fisher Scientific, Cat. No. V652020) for stable cell line generation with the following scheme: Three plasmids expressing miniG proteins were used in the project: The remaining plasmids (ETA-Rluc8 and SRE reporter plasmid ) used in this study are documented elsewhere. .. Flp-In T-REx 293 Cell Line was co-transfected with 200 ng of pcDNA5/FRT/TO plasmids encoding the various Adgrl3 constructs and 1,800 ng of the pOG44 Flp-In Recombinant vector (Thermo Fisher Scientific, Cat. No. V600520) using Lipofectamine 2000 (Thermo Fisher Scientific, Cat. No. 11668027) according to the manufacturer’s protocol.

Expressing:

Article Title: Direct tensile force activates Adgrl3 in a tethered agonist-dependent manner
Article Snippet: Plasmid construction was made either by restriction enzyme digestion followed by ligation or by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB, Cat. No. E2621L). .. All sequences were confirmed with Eurofins Genomics DNA sequencing service. cDNAs encoding SNAPf-tagged Adgrl3, Adgrl3-TM1, Adgrl3 LM>AA, and Adgrl3 T>G, were inserted into pcDNA5/FRT/TO (Thermo Fisher Scientific, Cat. No. V652020) for stable cell line generation with the following scheme: Three plasmids expressing miniG proteins were used in the project: The remaining plasmids (ETA-Rluc8 and SRE reporter plasmid ) used in this study are documented elsewhere. .. Flp-In T-REx 293 Cell Line was co-transfected with 200 ng of pcDNA5/FRT/TO plasmids encoding the various Adgrl3 constructs and 1,800 ng of the pOG44 Flp-In Recombinant vector (Thermo Fisher Scientific, Cat. No. V600520) using Lipofectamine 2000 (Thermo Fisher Scientific, Cat. No. 11668027) according to the manufacturer’s protocol.

Plasmid Preparation:

Article Title: Direct tensile force activates Adgrl3 in a tethered agonist-dependent manner
Article Snippet: Plasmid construction was made either by restriction enzyme digestion followed by ligation or by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB, Cat. No. E2621L). .. All sequences were confirmed with Eurofins Genomics DNA sequencing service. cDNAs encoding SNAPf-tagged Adgrl3, Adgrl3-TM1, Adgrl3 LM>AA, and Adgrl3 T>G, were inserted into pcDNA5/FRT/TO (Thermo Fisher Scientific, Cat. No. V652020) for stable cell line generation with the following scheme: Three plasmids expressing miniG proteins were used in the project: The remaining plasmids (ETA-Rluc8 and SRE reporter plasmid ) used in this study are documented elsewhere. .. Flp-In T-REx 293 Cell Line was co-transfected with 200 ng of pcDNA5/FRT/TO plasmids encoding the various Adgrl3 constructs and 1,800 ng of the pOG44 Flp-In Recombinant vector (Thermo Fisher Scientific, Cat. No. V600520) using Lipofectamine 2000 (Thermo Fisher Scientific, Cat. No. 11668027) according to the manufacturer’s protocol.

Purification:

Article Title: A new species of Athelges ( Crustacea , Isopoda , Epicaridea , Bopyridae ) from Japan, with morphological and DNA barcode data
Article Snippet: The PCR products were purified using the ExoSAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA). .. Purified PCR products were submitted to the DNA sequencing service of Eurofins Genomics Inc (Tokyo, Japan). ..

Article Title: Identification and Characterization of Antiyeast Organic Acids Produced by Lactiplantibacillus plantarum 3121M0s Derived from Mongolian Traditional Fermented Milk, Airag
Article Snippet: .. The resulting PCR amplicon was extracted using MagExtractor DNA Fragment Purification Kit (Toyobo Co., Ltd.; Osaka, Japan) after agarose gel electrophoresis, and then submitted to the DNA sequencing service at Eurofins Genomics K.K. (Tokyo, Japan). ..

Agarose Gel Electrophoresis:

Article Title: Identification and Characterization of Antiyeast Organic Acids Produced by Lactiplantibacillus plantarum 3121M0s Derived from Mongolian Traditional Fermented Milk, Airag
Article Snippet: .. The resulting PCR amplicon was extracted using MagExtractor DNA Fragment Purification Kit (Toyobo Co., Ltd.; Osaka, Japan) after agarose gel electrophoresis, and then submitted to the DNA sequencing service at Eurofins Genomics K.K. (Tokyo, Japan). ..



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(A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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(A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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(A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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Image Search Results


(A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus DNA ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense sequence (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).

Journal: Bio-protocol

Article Title: Plasmid Curing of Pseudoalteromonas haloplanktis TAC125 Using Homologous Recombination and PTasRNA Gene Silencing

doi: 10.21769/BioProtoc.5687

Figure Lengend Snippet: (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus DNA ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense sequence (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).

Article Snippet: DNA sequencing service (e.g., Eurofins Genomics, Ebersberg, Germany) 7.

Techniques: Agarose Gel Electrophoresis, Plasmid Preparation, Negative Control, Cloning, Sequencing, Marker